mouse recombinant ccl8  (MedChemExpress)

Journal: Cell Death & Disease

Article Title: TREM2 facilitates gastric cancer progression and immune evasion via inhibiting TRIM21-mediated STAT1 degradation in tumor-associated macrophages

doi: 10.1038/s41419-025-08198-4

Figure Lengend Snippet: A , B KEGG and GSEA analysis between shTREM2 TAMs and Scramble TAMs. C Cytokines and chemokines-related genes regulated by TREM2 in RNA-seq data. D Cytokines and chemokines screening for the above genes depicted by venn diagram. ( E ) Volcano plots showing DEGs in the shTREM2 TAMs compared with scramble TAMs. |log2FC | > 1, p -value < 0.05. F Correlation analysis between TREM2 and CCL8 (upper panel) and PD-L1 (bottom panel) from TISIDB. G mRNA expression of CCL8 in 56-paired GC tumor tissues and adjacent tissues. H Representative images of CCL8 expression in paired tumors and adjacent tissues from 14 GC patients using western blot. I Representative images of the mIF staining of macrophage CCL8 and TREM2 in GC tumor tissues. J Percentage of CCL8 + macrophage based on TREM2 expression. K Correlation between macrophage CCL8 and TREM2 expression in GC tissues. Data are presented as mean ± SD. Statistical significance was analyzed using a Student’s t -test. *** < 0.001, **** < 0.0001.

Article Snippet: For the in vivo CCL8 recuse experiment, C57BL/6 mice with GC tumors were injected with 200 μg of mouse recombinant CCL8 (Cat. No. HY-P7239; MCE) or isotype control twice a week for two weeks.

Techniques: RNA Sequencing, Expressing, Western Blot, Staining

Journal: Cell Death & Disease

Article Title: TREM2 facilitates gastric cancer progression and immune evasion via inhibiting TRIM21-mediated STAT1 degradation in tumor-associated macrophages

doi: 10.1038/s41419-025-08198-4

Figure Lengend Snippet: A – D EdU and Colony formation assays for cell proliferation of GC cells cultured by conditioned media from shTREM2 TAMs, with or without recombinant CCL8. E – H Transwell and wound healing assays were used to test cellular invasion and migration of GC cells cultured by conditioned media from shTREM2 TAMs, with or without recombinant CCL8. I Gross appearance of subcutaneous GC tumors from indicated groups. J , K The tumor weight and tumor volume of each group at the end point. Data are presented as mean ± SD. Statistical significance was analyzed using one-way ANOVA test. ** < 0.01, *** < 0.001, **** < 0.0001.

Article Snippet: For the in vivo CCL8 recuse experiment, C57BL/6 mice with GC tumors were injected with 200 μg of mouse recombinant CCL8 (Cat. No. HY-P7239; MCE) or isotype control twice a week for two weeks.

Techniques: Cell Culture, Recombinant, Migration

Journal: Cell Death & Disease

Article Title: TREM2 facilitates gastric cancer progression and immune evasion via inhibiting TRIM21-mediated STAT1 degradation in tumor-associated macrophages

doi: 10.1038/s41419-025-08198-4

Figure Lengend Snippet: A Venn diagram presenting the overlapping potential unique TREM2-interacting proteins identified through LC-MS/MS analysis. B List of the top 10 potential unique proteins that interact with TREM2. C Silver staining of the gel. D , E Co-IP of endogenous TREM2 with STAT1 in TAMs. F Representative IF images of the colocalization of TREM2 and STAT1 protein. G GST pull-down assays were conducted to investigate the direct binding between TREM2 and STAT1. ( H ) Western blot of TREM2 and phosphorylated/non-phosphorylated STAT1, PD-L1, and CCL8 in the indicated TAMs. I The level of CCL8 was evaluated using western blot and ELISA in indicated groups. J The level of PD-L1 was evaluated using western blot and RT-qPCR in indicated groups. K Schematic of putative STAT1-binding sites in the CCL8 gene promoter region and the sequence logo and frequency matrix of STAT1. L ChIP PCR to investigate the binding of STAT1 to the CCL8 promoter sequences in indicated TAMs. M Relative luciferase activities of reporters containing full-length or fragments of the CCL8 promoter. N Relative luciferase activities of different reporters containing mutated sequences of the CCL8 promoter in the indicated TAMs. Data are presented as mean ± SD. Statistical significance was analyzed using a Student’s t -test and one-way ANOVA test. ** < 0.01, *** < 0.001, **** < 0.0001, ns, not significant.

Article Snippet: For the in vivo CCL8 recuse experiment, C57BL/6 mice with GC tumors were injected with 200 μg of mouse recombinant CCL8 (Cat. No. HY-P7239; MCE) or isotype control twice a week for two weeks.

Techniques: Liquid Chromatography with Mass Spectroscopy, Silver Staining, Co-Immunoprecipitation Assay, Binding Assay, Western Blot, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Sequencing, Luciferase

Journal: Cell Death & Disease

Article Title: TREM2 facilitates gastric cancer progression and immune evasion via inhibiting TRIM21-mediated STAT1 degradation in tumor-associated macrophages

doi: 10.1038/s41419-025-08198-4

Figure Lengend Snippet: A Western blot of the protein and phosphorylation level of STAT1 in indicated TAMs. B Western blot of phosphorylated and non-phosphorylated STAT1, CCL8, and PD-L1 in the indicated TAMs. C , D Co-IP of exogenous SYK with STAT1 in HEK-293T. E Co-IP of endogenous SYK with STAT1 in TAMs. F Co-IP of exogenous phosphorylation of STAT1 in HEK-293T. G Co-IP of interaction between STAT1 and SYK in TREM2 overexpression and knockdown TAMs. H GST pull-down assays were conducted to investigate the direct binding between STAT1 and SYK with or without TREM2. I Co-IP of phosphorylation of STAT1 in TREM2 overexpression and knockdown TAMs. J Western blot of cytoplasmic and nuclear phosphorylated and non-phosphorylated STAT1 in TREM2 overexpression and knockdown TAMs. K , L IF images of the colocalization of TREM2 and p-STAT1 protein. M Schematic diagram showing TREM2 recruits STAT1 and promotes its phosphorylation through SYK, which, in turn, upregulates CCL8 transcription in TAMs. N , O Representative mIF images of fresh GC tumor sections. Data are presented as mean ± SD. Statistical significance was analyzed using a Student’s t -test. ** < 0.01, *** < 0.001.

Article Snippet: For the in vivo CCL8 recuse experiment, C57BL/6 mice with GC tumors were injected with 200 μg of mouse recombinant CCL8 (Cat. No. HY-P7239; MCE) or isotype control twice a week for two weeks.

Techniques: Western Blot, Phospho-proteomics, Co-Immunoprecipitation Assay, Over Expression, Knockdown, Binding Assay

Journal: Journal of Translational Medicine

Article Title: Single-cell RNA sequencing reveals recruitment of the M2-like CCL8 high macrophages in Lewis lung carcinoma-bearing mice following hypofractionated radiotherapy

doi: 10.1186/s12967-024-05118-6

Figure Lengend Snippet: Single-cell transcriptomic landscape of subcutaneous LLC tumors at early-stage following hypofractionated radiotherapy. A Overview of the experimental design for single-cell RNA sequencing. B Uniform Manifold Approximation (UMAP) plot showing the unsupervised clusters of 54883 single cells and annotated cell types. C The proportion of each cell types in LLC tumors treated with or without radiation. For B and C, each color represents the same cell type. D Feature plot and E Dot plot showing marker genes. F Heatmap showing serum chemokines level in LLC murine models 72 h post-treatment. Each row in the heatmap has been scaled. G The concentrations of CCL2, CCL7, CCL8, and CSF1 in serum measured by ELISA (n = 5/group). Data are presented as mean ± SD with student unpaired t test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant

Article Snippet: For proliferation assay and colony formation assay, the LLC cell line was treated with recombinant CCL8 (MedChemExpress, Cat#: HY-P7771) at 0, 5 ng/ml, 20 ng/ml.

Techniques: RNA Sequencing, Marker, Enzyme-linked Immunosorbent Assay

Journal: Journal of Translational Medicine

Article Title: Single-cell RNA sequencing reveals recruitment of the M2-like CCL8 high macrophages in Lewis lung carcinoma-bearing mice following hypofractionated radiotherapy

doi: 10.1186/s12967-024-05118-6

Figure Lengend Snippet: Identification of M2-like Ccl8 high Macrophages in the LLC-bearing murine model. A UMAP plot showing the annotation of macrophage populations in LLC tumors. B Feature plot and C Dotplot showing marker genes of macrophage populations. D Cell proportion of each cell type in NT and RT groups. E Heatmap displaying scores of M1, M2, angiogenesis, phagocytosis for each macrophage population. F Correlation analysis of CCL8 and CD163 expression level in TCGA-LUAD datasets (Spearman’s rho value = 0.542, p < 0.001). G The Gene Ontology (GO) enrichment analysis of each macrophage populations. H Heatmap displaying transcription factors activity of the Mac_Ccl8, Mac_Hmox1, Mono_Cxcl3, and Mono_Plac8 populations. I Development trajectory of macrophages populations predicted by Monocle2. J The cell density and K gene expression patterns along with the pseudotime,

Article Snippet: For proliferation assay and colony formation assay, the LLC cell line was treated with recombinant CCL8 (MedChemExpress, Cat#: HY-P7771) at 0, 5 ng/ml, 20 ng/ml.

Techniques: Marker, Expressing, Activity Assay, Gene Expression

Journal: Journal of Translational Medicine

Article Title: Single-cell RNA sequencing reveals recruitment of the M2-like CCL8 high macrophages in Lewis lung carcinoma-bearing mice following hypofractionated radiotherapy

doi: 10.1186/s12967-024-05118-6

Figure Lengend Snippet: Hypofractionated radiotherapy promoted the crosstalk between the Mac_Ccl8 and lymphocytes. A UMAP plot showing the annotation of lymphocyte populations. B Feature plot and C Dot plot showing marker genes of lymphocyte populations. D The proportion of each lymphocyte population in two groups. E Violin plots displaying the expression level of immune checkpoint ligand genes in each lymphocyte populations of NT and RT groups. F Circle plots showing number of interactions in two groups inferred by CellChat. G The comparison of cellular communication probability from Mac_Ccl8 to T and NK cells in between two groups. H Chord plot displaying the upregulated signaling pathways in the RT group relative to the NT group. I Heatmap of the differential interaction strength of the GALECTIN signaling pathway between two groups

Article Snippet: For proliferation assay and colony formation assay, the LLC cell line was treated with recombinant CCL8 (MedChemExpress, Cat#: HY-P7771) at 0, 5 ng/ml, 20 ng/ml.

Techniques: Marker, Expressing, Comparison, Protein-Protein interactions

Journal: Journal of Translational Medicine

Article Title: Single-cell RNA sequencing reveals recruitment of the M2-like CCL8 high macrophages in Lewis lung carcinoma-bearing mice following hypofractionated radiotherapy

doi: 10.1186/s12967-024-05118-6

Figure Lengend Snippet: Hypofractionated radiotherapy reprograms CCL8 high macrophages through the CCL signaling pathway. A Volcano plot showing differentially expressed genes of the Mac_Ccl8 between the NT and RT groups. Adjusted p value < 0.05, two-sided Wilcoxon test. B Violin plots comparing the expression of Ccl2, Ccl3, Ccl4, Ccl7, Ccl8, and Ccl12 in the NT and RT groups. Unpaired two-sided Wilcoxon test. C Representative examples of multiplex immunofluorescent labeling CD206 and CCL8. Green, CD206; Red, CCL8; Blue, DAPI. D Bar plots showing the GO enrichment analysis of upregulation and downregulation genes in the RT group. E Differences in IFN-Gamma and TNF pathways activity between two groups inferred by GSEA. F Cell–cell communication network between myeloid populations and LLC cells. G River plot displaying communication patterns of different cell types. H Chord plot (top) and heatmap (bottom) showing communication network of CCL signaling pathway in different cell types. I Weighted network analysis of differential interaction strength of signals in the Mac_Ccl8 population between two groups. J The comparison of cellular communication probability from Mac_Ccl8 to other myeloid populations between two groups. K Chord plot displaying the upregulated signaling pathways in the RT group relative to the NT group. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant

Article Snippet: For proliferation assay and colony formation assay, the LLC cell line was treated with recombinant CCL8 (MedChemExpress, Cat#: HY-P7771) at 0, 5 ng/ml, 20 ng/ml.

Techniques: Expressing, Multiplex Assay, Labeling, Activity Assay, Comparison, Protein-Protein interactions

Journal: Journal of Translational Medicine

Article Title: Single-cell RNA sequencing reveals recruitment of the M2-like CCL8 high macrophages in Lewis lung carcinoma-bearing mice following hypofractionated radiotherapy

doi: 10.1186/s12967-024-05118-6

Figure Lengend Snippet: Hypofractionated radiotherapy promotes M2-like Ccl8 high macrophages infiltration and leads to poor prognosis. A Kaplan–Meier plots showing worse clinical prognosis in the LUAD and HNSC patients with the higher expression level of the Mac_Ccl8 signature. HR, hazard ratio. B Schematic diagram of the combination treatment with hypofractionated radiotherapy and the Bindarit. The intraperitoneally administration of Bindarit began from day 5 to day 11 post-tumor injection, and radiation treatment was initiated from day 7 to day 9 post-tumor injection. C Growth curves of tumors in LLC-bearing mice in the indicated treatment groups (n = 5 mice/group). Data are presented as mean ± SD with two-way ANOVA test. D Representative examples in the indicated treatment groups of multiplex immunofluorescent labeling F4/80, CD206 and CCL8. Yellow, F4/80, Green, CD206; Red, CCL8; Blue, DAPI. E Percentages of M1 and M2 macrophages in the specific treatment groups analyzed by flow cytometry (n = 3/group). F Representative flow cytometry panels showing M2 macrophages (top) and M1 macrophages (bottom). BI, Bindarit; RT, Radiation therapy; RT + BI, the combination therapy of the radiation and the Bindarit

Article Snippet: For proliferation assay and colony formation assay, the LLC cell line was treated with recombinant CCL8 (MedChemExpress, Cat#: HY-P7771) at 0, 5 ng/ml, 20 ng/ml.

Techniques: Expressing, Injection, Multiplex Assay, Labeling, Flow Cytometry

Journal: Journal of Translational Medicine

Article Title: Single-cell RNA sequencing reveals recruitment of the M2-like CCL8 high macrophages in Lewis lung carcinoma-bearing mice following hypofractionated radiotherapy

doi: 10.1186/s12967-024-05118-6

Figure Lengend Snippet: Single-cell transcriptomic landscape of subcutaneous LLC tumors at early-stage following hypofractionated radiotherapy. A Overview of the experimental design for single-cell RNA sequencing. B Uniform Manifold Approximation (UMAP) plot showing the unsupervised clusters of 54883 single cells and annotated cell types. C The proportion of each cell types in LLC tumors treated with or without radiation. For B and C, each color represents the same cell type. D Feature plot and E Dot plot showing marker genes. F Heatmap showing serum chemokines level in LLC murine models 72 h post-treatment. Each row in the heatmap has been scaled. G The concentrations of CCL2, CCL7, CCL8, and CSF1 in serum measured by ELISA (n = 5/group). Data are presented as mean ± SD with student unpaired t test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant

Article Snippet: Recombinant CCL8 protein was purchased from MCE (Cat. #: HY-P7771).

Techniques: RNA Sequencing, Marker, Enzyme-linked Immunosorbent Assay

Journal: Journal of Translational Medicine

Article Title: Single-cell RNA sequencing reveals recruitment of the M2-like CCL8 high macrophages in Lewis lung carcinoma-bearing mice following hypofractionated radiotherapy

doi: 10.1186/s12967-024-05118-6

Figure Lengend Snippet: Identification of M2-like Ccl8 high Macrophages in the LLC-bearing murine model. A UMAP plot showing the annotation of macrophage populations in LLC tumors. B Feature plot and C Dotplot showing marker genes of macrophage populations. D Cell proportion of each cell type in NT and RT groups. E Heatmap displaying scores of M1, M2, angiogenesis, phagocytosis for each macrophage population. F Correlation analysis of CCL8 and CD163 expression level in TCGA-LUAD datasets (Spearman’s rho value = 0.542, p < 0.001). G The Gene Ontology (GO) enrichment analysis of each macrophage populations. H Heatmap displaying transcription factors activity of the Mac_Ccl8, Mac_Hmox1, Mono_Cxcl3, and Mono_Plac8 populations. I Development trajectory of macrophages populations predicted by Monocle2. J The cell density and K gene expression patterns along with the pseudotime,

Article Snippet: Recombinant CCL8 protein was purchased from MCE (Cat. #: HY-P7771).

Techniques: Marker, Expressing, Activity Assay, Gene Expression

Journal: Journal of Translational Medicine

Article Title: Single-cell RNA sequencing reveals recruitment of the M2-like CCL8 high macrophages in Lewis lung carcinoma-bearing mice following hypofractionated radiotherapy

doi: 10.1186/s12967-024-05118-6

Figure Lengend Snippet: Hypofractionated radiotherapy promoted the crosstalk between the Mac_Ccl8 and lymphocytes. A UMAP plot showing the annotation of lymphocyte populations. B Feature plot and C Dot plot showing marker genes of lymphocyte populations. D The proportion of each lymphocyte population in two groups. E Violin plots displaying the expression level of immune checkpoint ligand genes in each lymphocyte populations of NT and RT groups. F Circle plots showing number of interactions in two groups inferred by CellChat. G The comparison of cellular communication probability from Mac_Ccl8 to T and NK cells in between two groups. H Chord plot displaying the upregulated signaling pathways in the RT group relative to the NT group. I Heatmap of the differential interaction strength of the GALECTIN signaling pathway between two groups

Article Snippet: Recombinant CCL8 protein was purchased from MCE (Cat. #: HY-P7771).

Techniques: Marker, Expressing, Comparison, Protein-Protein interactions

Journal: Journal of Translational Medicine

Article Title: Single-cell RNA sequencing reveals recruitment of the M2-like CCL8 high macrophages in Lewis lung carcinoma-bearing mice following hypofractionated radiotherapy

doi: 10.1186/s12967-024-05118-6

Figure Lengend Snippet: Hypofractionated radiotherapy reprograms CCL8 high macrophages through the CCL signaling pathway. A Volcano plot showing differentially expressed genes of the Mac_Ccl8 between the NT and RT groups. Adjusted p value < 0.05, two-sided Wilcoxon test. B Violin plots comparing the expression of Ccl2, Ccl3, Ccl4, Ccl7, Ccl8, and Ccl12 in the NT and RT groups. Unpaired two-sided Wilcoxon test. C Representative examples of multiplex immunofluorescent labeling CD206 and CCL8. Green, CD206; Red, CCL8; Blue, DAPI. D Bar plots showing the GO enrichment analysis of upregulation and downregulation genes in the RT group. E Differences in IFN-Gamma and TNF pathways activity between two groups inferred by GSEA. F Cell–cell communication network between myeloid populations and LLC cells. G River plot displaying communication patterns of different cell types. H Chord plot (top) and heatmap (bottom) showing communication network of CCL signaling pathway in different cell types. I Weighted network analysis of differential interaction strength of signals in the Mac_Ccl8 population between two groups. J The comparison of cellular communication probability from Mac_Ccl8 to other myeloid populations between two groups. K Chord plot displaying the upregulated signaling pathways in the RT group relative to the NT group. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant

Article Snippet: Recombinant CCL8 protein was purchased from MCE (Cat. #: HY-P7771).

Techniques: Expressing, Multiplex Assay, Labeling, Activity Assay, Comparison, Protein-Protein interactions

Journal: Journal of Translational Medicine

Article Title: Single-cell RNA sequencing reveals recruitment of the M2-like CCL8 high macrophages in Lewis lung carcinoma-bearing mice following hypofractionated radiotherapy

doi: 10.1186/s12967-024-05118-6

Figure Lengend Snippet: Hypofractionated radiotherapy promotes M2-like Ccl8 high macrophages infiltration and leads to poor prognosis. A Kaplan–Meier plots showing worse clinical prognosis in the LUAD and HNSC patients with the higher expression level of the Mac_Ccl8 signature. HR, hazard ratio. B Schematic diagram of the combination treatment with hypofractionated radiotherapy and the Bindarit. The intraperitoneally administration of Bindarit began from day 5 to day 11 post-tumor injection, and radiation treatment was initiated from day 7 to day 9 post-tumor injection. C Growth curves of tumors in LLC-bearing mice in the indicated treatment groups (n = 5 mice/group). Data are presented as mean ± SD with two-way ANOVA test. D Representative examples in the indicated treatment groups of multiplex immunofluorescent labeling F4/80, CD206 and CCL8. Yellow, F4/80, Green, CD206; Red, CCL8; Blue, DAPI. E Percentages of M1 and M2 macrophages in the specific treatment groups analyzed by flow cytometry (n = 3/group). F Representative flow cytometry panels showing M2 macrophages (top) and M1 macrophages (bottom). BI, Bindarit; RT, Radiation therapy; RT + BI, the combination therapy of the radiation and the Bindarit

Article Snippet: Recombinant CCL8 protein was purchased from MCE (Cat. #: HY-P7771).

Techniques: Expressing, Injection, Multiplex Assay, Labeling, Flow Cytometry